Multiplex CRISPR Design

2017-01-20

Add an option to download all vector maps in a ZIP file. Fix a wording issue in the list of all maps.

2016-12-13

Combined the primer design and map construction into one tool. This should simplify the use of the tool. Also tweaked output to show which primers are used in which PCR reactions.

2016-12-12

Fixed titles and typos.

2016-11-30

Added three new vector maps.

2016-11-25

Updated page titles, links, and protocol information. Fixed a bug with the vector selection form. Updated primer names and sequences in the map construction tool.

2016-11-03

Fixed an issue with shifted annotations with the YAO promoter. Added two missing vector maps.

2016-09-13

Trimmed list of destination vectors available for map construction. Fixed sequence errors with pMOD_B2103b and pMOD_C2200 map construction. Added download links for protocols and backbones. Fixed a bug where filenames with spaces gave no output.

2016-07-04

Added new destination vectors. Fixed an issue with sequence placement in ribozyme systems. Map construction for pDIRECT and pTRANS vectors still under construction.

2016-04-26

Added SbfI site to map building output. Added option in primer design for no promoter as part of the assembly - for two specific destination vectors.

2016-04-15

Revision of the vector map construction script. Destination vectors added. Special cases not yet implemented.

2016-03-30

First draft of the vector map construction script uploaded.

2016-03-29

Some styling changes. Create a pane on the left with links to tools and sites.

2016-03-24

Finalized vector design. Styled a few elements. Still need to integrate vector design with primer design.

2016-03-18

Put the vector design script in place. Still needs to be set up to talk to the primer design script.

2016-03-08

First attempt at incporporating the tRNA and Ribozyme splicing systems into the script.

2016-03-07

Initial upload