Webtools for the Voytas Lab Plant Genome Engineering Toolkit

List of All Target Vectors

Click on the vector name to download a GenBank flat file containing the annotated vector map file. Click on the protocol name to download a ZIP file containing a description of the protocol and the protocol itself.

Alternately, you may download all maps in one ZIP file.

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Module A
Module B
Module C
Transformation Backbones
Direct Cloning Backbones
T-DNA with Cas9 Only

Module A

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Plasmid ID Gene Promoter Terminator Protocols
pMOD_A0000 None None None 5
pMOD_A0101 AtCas9 35S HSP 5
pMOD_A0102 AtCas9 AtUbi10 HSP 5
pMOD_A0103 AtCas9 CmYLCV HSP 5
pMOD_A0108 AtCas9 EC1.2 HSP 5
pMOD_A0201 AtCas9_D10A nickase 35S HSP 5
pMOD_A0202 AtCas9_D10A nickase AtUbi10 HSP 5
pMOD_A0208 AtCas9_D10A nickase EC1.2 HSP 5
pMOD_A0301 AtCas9_H840A nickase 35S HSP 5
pMOD_A0302 AtCas9_H840A nickase AtUbi10 HSP 5
pMOD_A0308 AtCas9_H840A nickase EC1.2 HSP 5
pMOD_A0401 AtCas9_dead (D10A + H840A) 35S HSP 5
pMOD_A0402 AtCas9_dead (D10A + H840A) AtUbi10 HSP 5
pMOD_A0408 AtCas9_dead (D10A + H840A) EC1.2 HSP 5
pMOD_A0501 Csy4-P2A-AtCas9 35S HSP 5
pMOD_A0502 Csy4-P2A-AtCas9 AtUbi10 HSP 5
pMOD_A0503 Csy4-P2A-AtCas9 CmYLCV HSP 5
pMOD_A0508 Csy4-P2A-AtCas9 EC1.2 HSP 5
pMOD_A0521 Csy4-P2A-AtCas9 GmUbi HSP 5
pMOD_A0601 Csy4-P2A-AtCas9_D10A nickase 35S HSP 5
pMOD_A0602 Csy4-P2A-AtCas9_D10A nickase AtUbi10 HSP 5
pMOD_A0608 Csy4-P2A-AtCas9_D10A nickase EC1.2 HSP 5
pMOD_A0701 Csy4-P2A-AtCas9_H840A nickase 35S HSP 5
pMOD_A0702 Csy4-P2A-AtCas9_H840A nickase AtUbi10 HSP 5
pMOD_A0708 Csy4-P2A-AtCas9_H840A nickase EC1.2 HSP 5
pMOD_A0801 Csy4-P2A-AtCas9_dead (D10A + H840A) 35S HSP 5
pMOD_A0802 Csy4-P2A-AtCas9_dead (D10A + H840A) AtUbi10 HSP 5
pMOD_A0808 Csy4-P2A-AtCas9_dead (D10A + H840A) EC1.2 HSP 5
pMOD_A0901 TREX2-P2A-AtCas9 35S HSP 5
pMOD_A0902 TREX2-P2A-AtCas9 AtUbi10 HSP 5
pMOD_A0908 TREX2-P2A-AtCas9 EC1.2 HSP 5
pMOD_A1001 TALEN 1 backbone with Esp3I ccdb cassette for repeat cloning 35S None (ends with P2A to be fused to TALEN_2) 1A, 5
pMOD_A1002 TALEN 1 backbone with Esp3I ccdb cassette for repeat cloning AtUbi10 None (ends with P2A to be fused to TALEN_2) 1A, 5
pMOD_A1010 TALEN 1 backbone with Esp3I ccdb cassette for repeat cloning ZmUbi None (ends with P2A to be fused to TALEN_2) 1A, 5
pMOD_A1110 TaCas9 ZmUbi HSP 5
pMOD_A1111 TaCas9 OsAct1 HSP 5
pMOD_A1210 TaCas9_D10A nickase ZmUbi HSP 5
pMOD_A1310 TaCas9_H840A nickase ZmUbi HSP 5
pMOD_A1410 TaCas9_dead (D10A + H840A) ZmUbi HSP 5
pMOD_A1510 Csy4-P2A-TaCas9 ZmUbi HSP 5
pMOD_A1511 Csy4-P2A-TaCas9 OsAct1 HSP 5
pMOD_A1610 Csy4-P2A-TaCas9_D10A nickase ZmUbi HSP 5
pMOD_A1710 Csy4-P2A-TaCas9_H840A nickase ZmUbi HSP 5
pMOD_A1810 Csy4-P2A-TaCas9_dead (D10A + H840A) ZmUbi HSP 5
pMOD_A1910 TREX2-P2A-TaCas9 ZmUbi HSP 5
pMOD_A3001 GFP 35S HSP 5
pMOD_A3003 GFP CmYLCV HSP 5
pMOD_A3010 GFP ZmUbi HSP 5
pMOD_A3012 GFP PvUbi1 HSP 5
pMOD_A3013 GFP PvUbi2 HSP 5
pMOD_A3014 GFP Nos HSP 5
pMOD_A3101 TALE-VPR_1 backbone with ccdb for repeat cloning 35S None (ends with P2A to be fused to TALE-VPR_2) 1A, 5
pMOD_A3301 TALE-VP64_1 backbone with ccdb for repeat cloning 35S None (ends with P2A to be fused to TALE-VP64_2) 1A, 5
pMOD_A3501 dAtCas9_VPR 35S HSP 5
pMOD_A3601 dAtCas9_VP64 35S HSP 5
pMOD_A3701 Csy4-P2A-dAtCas9_VPR 35S HSP 5
pMOD_A3801 Csy4-P2A-dAtCas9_VP64 35S HSP 5
pMOD_A3910 dTaCas9_VPR ZmUbi HSP 5
pMOD_A4010 dTaCas9_VP64 ZmUbi HSP 5
pMOD_A4110 Csy4-P2A-dTaCas9_VPR ZmUbi HSP 5
pMOD_A4210 Csy4-P2A-dTaCas9_VP64 ZmUbi HSP 5

Module B

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Plasmid ID Gene Promoter Terminator Protocols
pMOD_B0000 None None None 5
pMOD_B2000 TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning None (begins with P2A to be fused to TALEN 1) HSP 1A, 5
pMOD_B2101 SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers 35S 35S 3A, 3S2, 5
pMOD_B2103 SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers CmYLCV 35S 3A, 3S2, 5
pMOD_B2103b SapI ccdb cassette (promoter in the backbone, not in the assembly) for cloning multiple gRNA protospacers with Csy4 spacers CmYLCV 35S 3A, 3S1, 3S2, 5
pMOD_B2109 SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers YAO 35S 3A, 3S2, 5
pMOD_B2112 SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers PvUbi1 35S 3A, 3S2, 5
pMOD_B2121 SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers GmUbi 35S 3A, 3S2, 5
pMOD_B2203 SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers - combinable with another array of multiple gRNA protospacers in MODULE C CmYLCV None (will be fused to gRNA array in MODULE C) 3S1, 3S2, 5
pMOD_B2301 SapI ccdb cassette for cloning multiple gRNA protospacers with tRNA spacers 35S 35S 3B, 3S2, 5
pMOD_B2303 SapI ccdb cassette for cloning multiple gRNA protospacers with tRNA spacers CmYLCV 35S 3B, 3S2, 5
pMOD_B2309 SapI ccdb cassette for cloning multiple gRNA protospacers with tRNA spacers YAO 35S 3B, 3S2, 5
pMOD_B2312 SapI ccdb cassette for cloning multiple gRNA protospacers with tRNA spacers PvUbi1 35S 3B, 3S2, 5
pMOD_B2403 SapI ccdb cassette for cloning multiple gRNA protospacers with ribozyme spacers CmYLCV 35S 3C, 3S2, 5
pMOD_B2515 Esp3I ccdb cassette for single gRNA spacer cloning AtU6 Pol III 2A, 5
pMOD_B2515b Esp3I ccdb cassette for single gRNA spacer cloning - structurally optimized gRNA scaffold AtU6 Pol III 2A, 5
pMOD_B2517 Esp3I ccdb cassette for single gRNA spacer cloning TaU3 Pol III 2A, 5
pMOD_B2518 Esp3I ccdb cassette for single gRNA spacer cloning TaU6 Pol III 2A, 5
pMOD_B2519 Esp3I ccdb cassette for single gRNA spacer cloning OsU3 Pol III 2A, 5
pMOD_B2520 Esp3I ccdb cassette for single gRNA spacer cloning OsU6 Pol III 2A, 5
pMOD_B2615 BsaI ccdb cassette for single gRNA spacer cloning AtU6 Pol III 2A, 5
pMOD_B3200 TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning None (begins with P2A to be fused to TALE-VPR_1) HSP 1A, 5
pMOD_B3400 TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning None (begins with P2A to be fused to TALE-VP64_1) HSP 1A, 5

Module C

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Plasmid ID Gene Promoter Terminator Protocols
pMOD_C0000 None (BaeI site for GT donor cloning) None None 4, 5
pMOD_C2200 SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers - combinable with another array of multiple gRNA protospacers in MODULE B or C None (will be fused to gRNA array in MODULE B) 35S 3S1, 3S2, 4, 5
pMOD_C2515 Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) AtU6 Pol III 2A, 4, 5
pMOD_C2516 Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) At7SL Pol III 2A, 4, 5
pMOD_C2516b Esp3I ccdb cassette for single gRNA spacer cloning - structurally optimized gRNA scaffold (+ BaeI site for GT donor cloning) At7SL Pol III 2A, 4, 5
pMOD_C2517 Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) TaU3 Pol III 2A, 4, 5
pMOD_C2518 Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) TaU6 Pol III 2A, 4, 5
pMOD_C2519 Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) OsU3 Pol III 2A, 4, 5
pMOD_C2520 Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) OsU6 Pol III 2A, 4, 5
pMOD_C2616 BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) At7SL Pol III 2A, 4, 5
pMOD_C2806 Csy4 (+ BaeI site for GT donor cloning) FMV pea rbcsE9 4, 5
pMOD_C2813 Csy4 (+ BaeI site for GT donor cloning) PvUbi2 pea rbcsE9 4, 5
pMOD_C2906 TREX2 (+ BaeI site for GT donor cloning) FMV pea rbcsE9 4, 5
pMOD_C3001 GFP (+ BaeI site for GT donor cloning) 35S pea rbcsE9 4, 5
pMOD_C3003 GFP (+ BaeI site for GT donor cloning) CmYLCV pea rbcsE9 4, 5
pMOD_C3006 GFP (+ BaeI site for GT donor cloning) FMV pea rbcsE9 4, 5
pMOD_C3007 GFP (+ BaeI site for GT donor cloning) M24 pea rbcsE9 4, 5
pMOD_C3010 GFP (+ BaeI site for GT donor cloning) ZmUbi pea rbcsE9 4, 5
pMOD_C3012 GFP (+ BaeI site for GT donor cloning) PvUbi1 pea rbcsE9 4, 5
pMOD_C3013 GFP (+ BaeI site for GT donor cloning) PvUbi2 pea rbcsE9 4, 5
pMOD_C3106 TALE-VPR_1 backbone with ccdb for repeat cloning FMV pea rbcsE9 1A, 4, 5
pMOD_C3306 TALE-VP64_1 backbone with ccdb for repeat cloning FMV pea rbcsE9 1A, 4, 5
pMOD_C4300 BaeI LacZ cassette for GT donor cloning None None 4, 5

Transformation Backbones

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Plasmid ID Vector Type Plant Selection Viral Replicon Protocols
pTRANS_100 Non-T-DNA None None 5
pTRANS_101 Non-T-DNA None BeYDV 5
pTRANS_102 Non-T-DNA None ToLCV 5
pTRANS_103 Non-T-DNA None WDV 5
pTRANS_110 Non-T-DNA 2x35S:hpt II None 5
pTRANS_120 Non-T-DNA 2x35S:npt II None 5
pTRANS_130 Non-T-DNA 2x35S:bar None 5
pTRANS_150 Non-T-DNA PvUbi2:hpt II None 5
pTRANS_160 Non-T-DNA PvUbi2:bar None 5
pTRANS_200 T-DNA None None 5
pTRANS_201 T-DNA None BeYDV 5
pTRANS_202 T-DNA None ToLCV 5
pTRANS_203 T-DNA None WDV 5
pTRANS_210 T-DNA 2x35S:hpt II None 5
pTRANS_211 T-DNA 2x35S:hpt II BeYDV 5
pTRANS_221 T-DNA 2x35S:npt II BeYDV 5
pTRANS_231 T-DNA 2x35S:bar BeYDV 5
pTRANS_212 T-DNA 2x35S:hpt II ToLCV 5
pTRANS_222 T-DNA 2x35S:npt II ToLCV 5
pTRANS_232 T-DNA 2x35S:bar ToLCV> 5
pTRANS_213 T-DNA 2x35S:hpt II WDV 5
pTRANS_223 T-DNA 2x35S:npt II WDV 5
pTRANS_233 T-DNA 2x35S:bar WDV 5
pTRANS_253 T-DNA PvUbi2:hpt II WDV 5
pTRANS_263 T-DNA PvUbi2:bar WDV 5
pTRANS_210d T-DNA 2x35S:hpt II None 5
pTRANS_220 T-DNA 2x35S:npt II None 5
pTRANS_220d T-DNA 2x35S:npt II None 5
pTRANS_230 T-DNA 2x35S:bar None 5
pTRANS_230d T-DNA 2x35S:bar None 5
pTRANS_240 T-DNA 2x35S:hpt II w/intron None 5
pTRANS_250d T-DNA PvUbi2:hpt II None 5
pTRANS_260d T-DNA PvUbi2:bar None 5

Direct Cloning Backbones

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Plasmid ID Vector Type Plant Selection Genome Engineering Reagent Protocols
pDIRECT_10A Non-T-DNA None 35S:AtCas9 + AtU6:gRNA 2B
pDIRECT_10C Non-T-DNA None 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_10E Non-T-DNA None 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers 3B, 3S2
pDIRECT_21A T-DNA 2x35S:hpt II 35S:AtCas9 + AtU6:gRNA 2B
pDIRECT_21B T-DNA 2x35S:hpt II 35S:AtCas9_dead + AtU6:gRNA 2B
pDIRECT_21C T-DNA 2x35S:hpt II 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_21D T-DNA 2x35S:hpt II 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_21J T-DNA 2x35S:hpt II 35S:TALEN 1B
pDIRECT_22A T-DNA 2x35S:npt II 35S:AtCas9 + AtU6:gRNA 2B
pDIRECT_22B T-DNA 2x35S:npt II 35S:AtCas9_dead + AtU6:gRNA 2B
pDIRECT_22C T-DNA 2x35S:npt II 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_22D T-DNA 2x35S:npt II 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_22J T-DNA 2x35S:npt II 35S:TALEN 1B
pDIRECT_23A T-DNA 2x35S:bar 35S:AtCas9 + AtU6:gRNA 2B
pDIRECT_23B T-DNA 2x35S:bar 35S:AtCas9_dead + AtU6:gRNA 2B
pDIRECT_23C T-DNA 2x35S:bar 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_23D T-DNA 2x35S:bar 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_23J T-DNA 2x35S:bar 35S:TALEN 1B
pDIRECT_25F T-DNA PvUbi2:hpt II ZmUbi:TaCas9 + TaU6:gRNA 2B
pDIRECT_25G T-DNA PvUbi2:hpt II ZmUbi:TaCas9_dead + TaU6:gRNA 2B
pDIRECT_25H T-DNA PvUbi2:hpt II ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_25I T-DNA PvUbi2:hpt II ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_25K T-DNA PvUbi2:hpt II ZmUbi:TALEN 1B
pDIRECT_26F T-DNA PvUbi2:bar ZmUbi:TaCas9 + TaU6:gRNA 2B
pDIRECT_26G T-DNA PvUbi2:bar ZmUbi:TaCas9_dead + TaU6:gRNA 2B
pDIRECT_26H T-DNA PvUbi2:bar ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_26I T-DNA PvUbi2:bar ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers 3A, 3S2
pDIRECT_26K T-DNA PvUbi2:bar ZmUbi:TALEN 1B
pDIRECT_37J T-DNA nos:hpt II 35S:TALEN 1B
pDIRECT_38J T-DNA nos:npt II 35S:TALEN 1B
pDIRECT_39J T-DNA nos:bar 35S:TALEN 1B

T-DNA with Cas9 Only

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Plasmid ID Cas9 Type Plant Selection
pAtCas9_1 AtCas9 2x35S:hpt II
pAtCas9_2 AtCas9 2x35S:npt II
pAtCas9_3 AtCas9 2x35S:bar
pAtCas9-D10A_1 AtCas9_D10A (nickase) 2x35S:hpt II
pAtCas9-D10A_2 AtCas9_D10A (nickase) 2x35S:npt II
pAtCas9-D10A_3 AtCas9_D10A (nickase) 2x35S:bar
pAtCas9-H840A_1 AtCas9_H840A (nickase) 2x35S:hpt II
pAtCas9-H840A_2 AtCas9_H840A (nickase) 2x35S:npt II
pAtCas9-H840A_3 AtCas9_H840A (nickase) 2x35S:bar
pAtCas9-dead_1 AtCas9_D10A+H840A (dead) 2x35S:hpt II
pAtCas9-dead_2 AtCas9_D10A+H840A (dead) 2x35S:npt II
pAtCas9-dead_3 AtCas9_D10A+H840A (dead) 2x35S:bar
pTaCas9_5 TaCas9 PvUbi2:hpt II
pTaCas9_6 TaCas9 PvUbi2:bar
pTaCas9-D10A_5 TaCas9_D10A (nickase) PvUbi2:hpt II
pTaCas9-D10A_6 TaCas9_D10A (nickase) PvUbi2:bar
pTaCas9-H840A_5 TaCas9_H840A (nickase) PvUbi2:hpt II
pTaCas9-H840A_6 TaCas9_H840A (nickase) PvUbi2:bar
pTaCas9-dead_5 TaCas9_D10A+H840A (dead) PvUbi2:hpt II
pTaCas9-dead_6 TaCas9_D10A+H840A (dead) PvUbi2:bar

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