Webtools for the Voytas Lab Plant Genome Engineering Toolkit
List of All Target Vectors
Click on the vector name to download a GenBank flat file containing the annotated vector map file. Click on the protocol name to download a ZIP file containing a description of the protocol and the protocol itself.
Alternately, you may download all maps in one ZIP file.
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Module A
Module B
Module C
Transformation Backbones
Direct Cloning Backbones
T-DNA with Cas9 Only
Module A
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Module B
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Plasmid ID |
Gene |
Promoter |
Terminator |
Protocols |
pMOD_B0000 |
None |
None |
None |
5 |
pMOD_B2000 |
TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning |
None (begins with P2A to be fused to TALEN 1) |
HSP |
1A, 5 |
pMOD_B2101 |
SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers |
35S |
35S |
3A, 3S2, 5 |
pMOD_B2103 |
SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers |
CmYLCV |
35S |
3A, 3S2, 5 |
pMOD_B2103b |
SapI ccdb cassette (promoter in the backbone, not in the assembly) for cloning multiple gRNA protospacers with Csy4 spacers |
CmYLCV |
35S |
3A, 3S1, 3S2, 5 |
pMOD_B2109 |
SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers |
YAO |
35S |
3A, 3S2, 5 |
pMOD_B2112 |
SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers |
PvUbi1 |
35S |
3A, 3S2, 5 |
pMOD_B2121 |
SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers |
GmUbi |
35S |
3A, 3S2, 5 |
pMOD_B2203 |
SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers - combinable with another array of multiple gRNA protospacers in MODULE C |
CmYLCV |
None (will be fused to gRNA array in MODULE C) |
3S1, 3S2, 5 |
pMOD_B2301 |
SapI ccdb cassette for cloning multiple gRNA protospacers with tRNA spacers |
35S |
35S |
3B, 3S2, 5 |
pMOD_B2303 |
SapI ccdb cassette for cloning multiple gRNA protospacers with tRNA spacers |
CmYLCV |
35S |
3B, 3S2, 5 |
pMOD_B2309 |
SapI ccdb cassette for cloning multiple gRNA protospacers with tRNA spacers |
YAO |
35S |
3B, 3S2, 5 |
pMOD_B2312 |
SapI ccdb cassette for cloning multiple gRNA protospacers with tRNA spacers |
PvUbi1 |
35S |
3B, 3S2, 5 |
pMOD_B2403 |
SapI ccdb cassette for cloning multiple gRNA protospacers with ribozyme spacers |
CmYLCV |
35S |
3C, 3S2, 5 |
pMOD_B2515 |
Esp3I ccdb cassette for single gRNA spacer cloning |
AtU6 |
Pol III |
2A, 5 |
pMOD_B2515b |
Esp3I ccdb cassette for single gRNA spacer cloning - structurally optimized gRNA scaffold |
AtU6 |
Pol III |
2A, 5 |
pMOD_B2517 |
Esp3I ccdb cassette for single gRNA spacer cloning |
TaU3 |
Pol III |
2A, 5 |
pMOD_B2518 |
Esp3I ccdb cassette for single gRNA spacer cloning |
TaU6 |
Pol III |
2A, 5 |
pMOD_B2519 |
Esp3I ccdb cassette for single gRNA spacer cloning |
OsU3 |
Pol III |
2A, 5 |
pMOD_B2520 |
Esp3I ccdb cassette for single gRNA spacer cloning |
OsU6 |
Pol III |
2A, 5 |
pMOD_B2615 |
BsaI ccdb cassette for single gRNA spacer cloning |
AtU6 |
Pol III |
2A, 5 |
pMOD_B3200 |
TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning |
None (begins with P2A to be fused to TALE-VPR_1) |
HSP |
1A, 5 |
pMOD_B3400 |
TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning |
None (begins with P2A to be fused to TALE-VP64_1) |
HSP |
1A, 5 |
Module C
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Plasmid ID |
Gene |
Promoter |
Terminator |
Protocols |
pMOD_C0000 |
None (BaeI site for GT donor cloning) |
None |
None |
4, 5 |
pMOD_C2200 |
SapI ccdb cassette for cloning multiple gRNA protospacers with Csy4 spacers - combinable with another array of multiple gRNA protospacers in MODULE B or C |
None (will be fused to gRNA array in MODULE B) |
35S |
3S1, 3S2, 4, 5 |
pMOD_C2515 |
Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) |
AtU6 |
Pol III |
2A, 4, 5 |
pMOD_C2516 |
Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) |
At7SL |
Pol III |
2A, 4, 5 |
pMOD_C2516b |
Esp3I ccdb cassette for single gRNA spacer cloning - structurally optimized gRNA scaffold (+ BaeI site for GT donor cloning) |
At7SL |
Pol III |
2A, 4, 5 |
pMOD_C2517 |
Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) |
TaU3 |
Pol III |
2A, 4, 5 |
pMOD_C2518 |
Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) |
TaU6 |
Pol III |
2A, 4, 5 |
pMOD_C2519 |
Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) |
OsU3 |
Pol III |
2A, 4, 5 |
pMOD_C2520 |
Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) |
OsU6 |
Pol III |
2A, 4, 5 |
pMOD_C2616 |
BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) |
At7SL |
Pol III |
2A, 4, 5 |
pMOD_C2806 |
Csy4 (+ BaeI site for GT donor cloning) |
FMV |
pea rbcsE9 |
4, 5 |
pMOD_C2813 |
Csy4 (+ BaeI site for GT donor cloning) |
PvUbi2 |
pea rbcsE9 |
4, 5 |
pMOD_C2906 |
TREX2 (+ BaeI site for GT donor cloning) |
FMV |
pea rbcsE9 |
4, 5 |
pMOD_C3001 |
GFP (+ BaeI site for GT donor cloning) |
35S |
pea rbcsE9 |
4, 5 |
pMOD_C3003 |
GFP (+ BaeI site for GT donor cloning) |
CmYLCV |
pea rbcsE9 |
4, 5 |
pMOD_C3006 |
GFP (+ BaeI site for GT donor cloning) |
FMV |
pea rbcsE9 |
4, 5 |
pMOD_C3007 |
GFP (+ BaeI site for GT donor cloning) |
M24 |
pea rbcsE9 |
4, 5 |
pMOD_C3010 |
GFP (+ BaeI site for GT donor cloning) |
ZmUbi |
pea rbcsE9 |
4, 5 |
pMOD_C3012 |
GFP (+ BaeI site for GT donor cloning) |
PvUbi1 |
pea rbcsE9 |
4, 5 |
pMOD_C3013 |
GFP (+ BaeI site for GT donor cloning) |
PvUbi2 |
pea rbcsE9 |
4, 5 |
pMOD_C3106 |
TALE-VPR_1 backbone with ccdb for repeat cloning |
FMV |
pea rbcsE9 |
1A, 4, 5 |
pMOD_C3306 |
TALE-VP64_1 backbone with ccdb for repeat cloning |
FMV |
pea rbcsE9 |
1A, 4, 5 |
pMOD_C4300 |
BaeI LacZ cassette for GT donor cloning |
None |
None |
4, 5 |
Transformation Backbones
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Direct Cloning Backbones
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Plasmid ID |
Vector Type |
Plant Selection |
Genome Engineering Reagent |
Protocols |
pDIRECT_10A |
Non-T-DNA |
None |
35S:AtCas9 + AtU6:gRNA |
2B |
pDIRECT_10C |
Non-T-DNA |
None |
35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_10E |
Non-T-DNA |
None |
35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers |
3B, 3S2 |
pDIRECT_21A |
T-DNA |
2x35S:hpt II |
35S:AtCas9 + AtU6:gRNA |
2B |
pDIRECT_21B |
T-DNA |
2x35S:hpt II |
35S:AtCas9_dead + AtU6:gRNA |
2B |
pDIRECT_21C |
T-DNA |
2x35S:hpt II |
35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_21D |
T-DNA |
2x35S:hpt II |
35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_21J |
T-DNA |
2x35S:hpt II |
35S:TALEN |
1B |
pDIRECT_22A |
T-DNA |
2x35S:npt II |
35S:AtCas9 + AtU6:gRNA |
2B |
pDIRECT_22B |
T-DNA |
2x35S:npt II |
35S:AtCas9_dead + AtU6:gRNA |
2B |
pDIRECT_22C |
T-DNA |
2x35S:npt II |
35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_22D |
T-DNA |
2x35S:npt II |
35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_22J |
T-DNA |
2x35S:npt II |
35S:TALEN |
1B |
pDIRECT_23A |
T-DNA |
2x35S:bar |
35S:AtCas9 + AtU6:gRNA |
2B |
pDIRECT_23B |
T-DNA |
2x35S:bar |
35S:AtCas9_dead + AtU6:gRNA |
2B |
pDIRECT_23C |
T-DNA |
2x35S:bar |
35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_23D |
T-DNA |
2x35S:bar |
35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_23J |
T-DNA |
2x35S:bar |
35S:TALEN |
1B |
pDIRECT_25F |
T-DNA |
PvUbi2:hpt II |
ZmUbi:TaCas9 + TaU6:gRNA |
2B |
pDIRECT_25G |
T-DNA |
PvUbi2:hpt II |
ZmUbi:TaCas9_dead + TaU6:gRNA |
2B |
pDIRECT_25H |
T-DNA |
PvUbi2:hpt II |
ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_25I |
T-DNA |
PvUbi2:hpt II |
ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_25K |
T-DNA |
PvUbi2:hpt II |
ZmUbi:TALEN |
1B |
pDIRECT_26F |
T-DNA |
PvUbi2:bar |
ZmUbi:TaCas9 + TaU6:gRNA |
2B |
pDIRECT_26G |
T-DNA |
PvUbi2:bar |
ZmUbi:TaCas9_dead + TaU6:gRNA |
2B |
pDIRECT_26H |
T-DNA |
PvUbi2:bar |
ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_26I |
T-DNA |
PvUbi2:bar |
ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers |
3A, 3S2 |
pDIRECT_26K |
T-DNA |
PvUbi2:bar |
ZmUbi:TALEN |
1B |
pDIRECT_37J |
T-DNA |
nos:hpt II |
35S:TALEN |
1B |
pDIRECT_38J |
T-DNA |
nos:npt II |
35S:TALEN |
1B |
pDIRECT_39J |
T-DNA |
nos:bar |
35S:TALEN |
1B |
T-DNA with Cas9 Only
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